A simple, Accurate, precise method was developed for the simultaneous estimation of the ivabradine and metoprolol in tablet dosage form. Chromatogram was run through Agilent C18 150 x 4.6 mm, 5m. Mobile phase containing Methanol:KH2PO4 taken in the ratio 50:50 was pumped through column at a flow rate of 0.8 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 260.0 nm. Retention time of metoprolol and ivabradine were found to be 2.210 min and 2.650 min. %RSD of the ivabradine and metoprolol were and found to be 0.4 and 0.6 respectively. %Recovery was obtained as 99.89% and 100.09% for ivabradine and metoprolol respectively. LOD, LOQ values obtained from regression equations of ivabradine and metoprolol were 0.02, 0.06 and 0.24, 0.74 respectively. Regression equation of ivabradine is y = 33598x + 1032 and y = 43436x + 2674 of metoprolol. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular quality control test in industries.
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